Research Paper Volume 11, Issue 13 pp 4641—4653

Age-associated changes of the intrinsic nervous system in relation with interstitial cells in the pre-weaning goat rumen

Figure 1. Histologic changes and the expression of PGP9.5 immuno-positive and its protein expression in the rumen wall of goat during pre-weaning (A a–i). Histologic changes of rumen wall with age (A–a, 5 days group; A–b, 40 days group; A–c, 70 days group). The distribution of PGP9.5-immunoreactivity in the rumen wall (A d–f) and MOD of PGP9.5-positive NFs in the mucosa (especially in the epithelium) with age (A–g, 5-day group; A–h, 40-day group; A–i,70-day group). The reverse changes of PGP9.5 positive NFs in the rumen wall (B) and in the rumen papilla (D) from new born to weaning. Western blot analysis of PGP9.5 in protein extracts of rumen segments from pre-weaning goats (N=4 per group) (C). β-actin was used as a loading control. Protein signals were quantified using densitometry analysis. Data are reported as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Newman-Keuls post-hoc test for multiple comparison (panel A) *P<0.05 vs 5d; **P<0.01 vs 5d. CM, the circular muscle layer; E, epithelium; P, papilla; L: lamina propria; LM, the longitudinal muscle layer; MP, the myenteric nerve plexus; CM, circular muscle layer; V, vessel. (Bar =160 μm in A a–c; Bar =100μm in A d–f; Bar =35μm in A g–i.)