Figure 1. Effects of different concentrations of DDP and TP on the porcine oocyte maturation. (A) Representative images of oocyte meiotic progression in control, DDP -exposed and TP-supplemented oocytes. Cumulus cell expansion of COCs and polar body extrusion of DOs were imaged by the confocal microscope. Scale bar, 350 μm (a-c); 120 μm (d-f); 50 μm (g-i). (B) The rates of polar body extrusion were recorded in control and different concentrations of DDP-exposed groups (2 μM, 5 μM, 10 μM and 20 μM) after culture for 44 h in vitro. (C) The rates of polar body extrusion were recorded in control and different concentrations of TP -supplemented groups (10 μM, 50 μM and 250μM) after culture for 44 h with 10 μM DDP in vitro. Data in were presented as mean percentage (mean ± SEM) of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.