Research Paper Volume 11, Issue 13 pp 4706—4719

Figure 1. Effects of different concentrations of DDP and TP on the porcine oocyte maturation. (A) Representative images of oocyte meiotic progression in control, DDP -exposed and TP-supplemented oocytes. Cumulus cell expansion of COCs and polar body extrusion of DOs were imaged by the confocal microscope. Scale bar, 350 μm (a-c); 120 μm (d-f); 50 μm (g-i). (B) The rates of polar body extrusion were recorded in control and different concentrations of DDP-exposed groups (2 μM, 5 μM, 10 μM and 20 μM) after culture for 44 h in vitro. (C) The rates of polar body extrusion were recorded in control and different concentrations of TP -supplemented groups (10 μM, 50 μM and 250μM) after culture for 44 h with 10 μM DDP in vitro. Data in were presented as mean percentage (mean ± SEM) of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.