Research Paper Volume 11, Issue 13 pp 4463—4477

Let-7f-5p suppresses Th17 differentiation via targeting STAT3 in multiple sclerosis

Figure 2. Let-7f-5p expression is decreased during MS and downregulated in Th17 cells. (A) CD4+ T cells were sorted from whole blood of multiple sclerosis (MS) patients (n = 6) and healthy controls (HC) (n = 6). The expression (let-7f-5p vs. U6) of let-7f-5p was analyzed by RT-PCR. (B) Naive CD4+ T cells were sorted from mouse spleens and maintained in Th17 differentiation medium. After 24, 48 and 96 h, the expression (let-7f-5p vs. U6) of let-7f-5p was analyzed by RT-PCR. (* p < 0.05, ** p < 0.01)