Research Paper Volume 11, Issue 15 pp 5334—5350

Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis

Figure 3. Establishment of co-expression network and identification of PDLSCs and lncRNA MEG3 was positively related with PDLSC osteogenic differentiation. (A) Expression of hPDLSC surface antigens including CD90, CD105, CD146, CD45, CD34, CD11b, CD19, STRO-1, and HLADR. (B) Expression of pPDLSC surface antigens including CD90, CD105, CD146, CD45, CD34, CD11b, CD19, STRO-1, and HLADR. (C) MEG3 was down-regulated in periodontitis tissues compared with heathy samples. (D) Expression of MEG3 mRNA in 0h, 24h, 7d, 14d and 21d after osteogenic induction. (E) Expression of MEG3 mRNA in PDLSCs treated with or without lenti-MEG3, sh-NC or sh-MEG3. (F) Western blot of osteoblast makers including Runx2, Osterix, Osteocalcin and Colla1. (G) ALP and Alizarin Red staining of PDLSCs treated with or without lenti-MEG3, sh-NC or sh-MEG3. ** P < 0.05, * P < 0.01, compared with the NC group.