Research Paper Volume 11, Issue 15 pp 5334—5350

Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis

Figure 4. LncRNA MEG3 regulated PDLSC osteogenic differentiation through miR-27a-3p. (A) MiR-27a-3p expression in PDLSCs was up-regulated in periodontitis periodontal tissues compared with healthy periodontal tissues. (B) Expression of miR-27a-3p in 0h, 24h, 7d, 14d and 21d after osteogenic induction. (C) MiR-27a-3p directly targeted MEG3. Co-transfection of lncRNA MEG3-3’UTR-wt and agomiR-27a-3p significantly reduced relative luciferase activity. (D) Expression of miR-27a-3p in group of agomiR-27a-3p or antagomiR-27a-3p transfection (E) Expression of MEG3 in group of agomiR-27a-3p or antagomiR-27a-3p transfection. (F) Western blot of osteoblast makers including Runx2, Osterix, Osteocalcin and Colla1 in group of agomiR-27a-3p or antagomiR-27a-3p transfection. (G) ALP and Alizarin Red staining of osteogenic activities in the agomiR-27a-3p group and antagomiR-27a-3p group. (H) Protein expression of certain genes in PI3k/Akt signaling pathway, including IGF1, IRS1, p-Akt, and Akt. ** P < 0.01, compared with the NC group