Figure 6. MiR-27a-3p regulated PDLSC osteogenic differentiation through targeting IGF1. (A) Western blot of Runx2, Osterix, Osteocalcin and Colla1 expression in PDLSCs with up-regulation or down-regulation of IGF1. (B–D) ALP and Alizarin Red staining of PDLSCs with different regulation of IGF1. (E) Protein expression of IGF1, IRS1, p-Akt and Akt in PDLSCs with different IGF1 regulation treatment. ** P < 0.01, compared with the NC group.