Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis

Yi Liu; Chunpeng Liu; Ankui Zhang; Shichang Yin; Ting Wang; Yan Wang; Meiming Wang; Yixin Liu; Qiaohui Ying; Jinrui Sun; Fulan Wei; Dongxu Liu; Chunling Wang; Shaohua Ge

doi:doi:10.18632/aging.102105

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Research Paper Volume 11, Issue 15 pp 5334—5350

Down-regulation of long non-coding RNA MEG3 suppresses osteogenic differentiation of periodontal ligament stem cells (PDLSCs) through miR-27a-3p/IGF1 axis in periodontitis

Figure 6. MiR-27a-3p regulated PDLSC osteogenic differentiation through targeting IGF1. (A) Western blot of Runx2, Osterix, Osteocalcin and Colla1 expression in PDLSCs with up-regulation or down-regulation of IGF1. (B–D) ALP and Alizarin Red staining of PDLSCs with different regulation of IGF1. (E) Protein expression of IGF1, IRS1, p-Akt and Akt in PDLSCs with different IGF1 regulation treatment. ** P < 0.01, compared with the NC group.