Figure 7. LncRNA MEG3/miR-27a-3p/IGF1 axis regulated PI3K/Akt signaling pathway to affect PDLSC osteogenic differentiation. (A, B) The results of western blot analysis indicated that overexpression of either lncRNA MEG3 or IGF1 promoted expression of PI3K/Akt-related proteins IRS1 and phosphorylated Akt (p-Akt), while agomiR-27a-3p and LY294002 blocked their expression. (C, D) Alizarin Red and ALP staining were also performed to validate this conclusion on cellular level. Increased calcium nodule formation and ALP activity were detected in lncRNA MEG3/IGF1 overexpression groups, while agomiR-27a-3p/LY294002 groups showed reverse results. ** P < 0.01, compared with the NC group. ## P＜0.01, compared with agomiR-NC group.