Research Paper Volume 11, Issue 15 pp 5351—5367

Long intergenic non-protein coding RNA 511 promotes the progression of osteosarcoma cells through sponging microRNA 618 to upregulate the expression of maelstrom

Figure 2. LINC00511 mediates the cell proliferation and apoptosis of HOS cells in vitro. (A) The relative expression level of LINC00511 was changed in HOS cells transfected with p-LINC00511- or LINC00511-targeting siRNA. P-LINC00511 is a LINC00511 overexpression vector, and its transfection induced a high level of LINC00511 expression. In contrast, transfection of specific LINC00511-targeting siRNA caused low expression levels of LINC00511 in HOS cells. (B) CCK-8 assays showed that the proliferation ability is changed in HOS cells with LINC00511 dysregulation. Up- or downregulation of LINC00511 was induced by pre-transfection with p-LINC00511 or specific siRNA. (C–D) Colony formation ability of HOS cells with altered expression of LINC00511. (E–F) Apoptosis rate of HOS cells with altered expression of LINC00511. *P < 0.05 compared to the NC group.