Research Paper Volume 11, Issue 15 pp 5351—5367

Long intergenic non-protein coding RNA 511 promotes the progression of osteosarcoma cells through sponging microRNA 618 to upregulate the expression of maelstrom

Figure 5. LINC00511 suppressed the expression of miR-618 in HOS cells. (A) The target relationship between miR-618 and LINC00511 was predicted by bioinformatics analysis (upper) and validated by dual-luciferase reporter assays (bottom). MiR-618 mimics significantly inhibit the fluorescence activity of the reporter vector carrying wild-type LINC00511, but not mutant LINC00511. *P<0.05 compared with the NC group. (B) The expression of miR-618 in OS tissues and adjacent tissues was measured using qRT-PCR. *P<0.05 compared to the adjacent group. (C) Downregulated expression of miR-618 in OS cell lines detected by qRT-PCR analysis. *P<0.05 compared with the noncancerous osteoblast cell line hFOB 1.19. (D) Expression of miR-618 in HOS cells is inhibited by LINC00511. *P<0.05 compared to the NC group.