Figure 7. The expression of MAEL is mediated by both miR-618 and LINC00511. (A) Binding sites between miR-618 and MAEL were predicted by TargetScanHuman7.2. Then, the target relationship between miR-618 and MAEL was validated by a dual-luciferase reporter assay. MiR-618 mimics significantly inhibited the expression of luciferase reporter vectors carrying the wild-type MAEL sequence but not the mutated MAEL sequence. *P<0.05 compared to the NC group. (B) A qRT-PCR assay shows that MAEL mRNA expression was higher in OS tissues than in corresponding normal tissues. *P<0.05 compared to the adjacent group. (C–D) MAEL protein expression levels were detected in OS tissues and adjacent normal tissues by Western blot assay (N=10). (E–F) MAEL mRNA (E) and protein (F) expression levels in OS cell lines. *P<0.05 and **P<0.01 compared to the noncancerous osteoblast cell line hFOB 1.19. (G–H) MAEL mRNA (G) and protein (H) expression levels in HOS cells transfected with NC, p-LINC00511, siRNA-LINC00511, and miR-618 inhibitor or mimics were tested by qRT-PCR and Western blot assays. *P<0.05 compared to the NC group.