Research Paper Volume 11, Issue 15 pp 5351—5367

Long intergenic non-protein coding RNA 511 promotes the progression of osteosarcoma cells through sponging microRNA 618 to upregulate the expression of maelstrom

Figure 7. The expression of MAEL is mediated by both miR-618 and LINC00511. (A) Binding sites between miR-618 and MAEL were predicted by TargetScanHuman7.2. Then, the target relationship between miR-618 and MAEL was validated by a dual-luciferase reporter assay. MiR-618 mimics significantly inhibited the expression of luciferase reporter vectors carrying the wild-type MAEL sequence but not the mutated MAEL sequence. *P<0.05 compared to the NC group. (B) A qRT-PCR assay shows that MAEL mRNA expression was higher in OS tissues than in corresponding normal tissues. *P<0.05 compared to the adjacent group. (C–D) MAEL protein expression levels were detected in OS tissues and adjacent normal tissues by Western blot assay (N=10). (E–F) MAEL mRNA (E) and protein (F) expression levels in OS cell lines. *P<0.05 and **P<0.01 compared to the noncancerous osteoblast cell line hFOB 1.19. (G–H) MAEL mRNA (G) and protein (H) expression levels in HOS cells transfected with NC, p-LINC00511, siRNA-LINC00511, and miR-618 inhibitor or mimics were tested by qRT-PCR and Western blot assays. *P<0.05 compared to the NC group.