Research Paper Volume 11, Issue 15 pp 5368—5388

Downregulation of microRNA-218 is cardioprotective against cardiac fibrosis and cardiac function impairment in myocardial infarction by binding to MITF

Figure 2. Downregulated miR-218 expression alleviates pathological damage of cardiac tissues in MI rat models. (A) the ratio of heart to body weight, n = 6; (B) serum levels of LDH and CPK examined by the biochemical analyzer, n = 6; (C) HE staining showing pathological changes of cardiac tissues (× 200), n = 3; (D) ultrastructure of cardiomyocytes observed by transmission electron microscope (× 5000), n = 3; (E) TTC staining showing the infarct size of rats, n = 3; (F) TUNEL staining showing the apoptotic index of cardiomyocytes (× 400), n = 3; (G) Western blot assay showing protein levels of apoptosis-related factors Bax and Bcl-2 in cardiac tissues, n = 3; *versus the sham group, P < 0.05; #versus the MI group, P < 0.05; Data were analyzed by one-way ANOVA. Pairwise comparison after ANOVA was performed by LSD-t. MI, myocardial infarction; miR-218, microRNA-218; LDH, lactate dehydrogenase; CPK, creatine phosphokinase.