Research Paper Volume 11, Issue 15 pp 5368—5388

Downregulation of microRNA-218 is cardioprotective against cardiac fibrosis and cardiac function impairment in myocardial infarction by binding to MITF

Figure 4. Downregulated miR-218 expression reduces oxidative stress and inflammatory injury in MI rats. (A) the levels of MDA and the activity of GSH-Px and SOD determined by the spectrocolorimetric method, n = 3; (B) the mRNA expression of IL-1β, IL-6 and TNF-α detected by RT-qPCR, n = 3; (C) the serum levels of IL-1β, IL-6 and TNF-α detected by ELISA, n = 6; *versus the sham group, P < 0.05; #versus the MI group, P < 0.05; Data were analyzed by one-way ANOVA. Pairwise comparison after ANOVA was performed by LSD-t. MI, myocardial infarction; miR-218, microRNA-218; ELISA, enzyme-linked immunosorbent assay; IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; MDA, malondialdehyde; GSH-Px, glutathione peroxidase; SOD, superoxide dismutase; RT-qPCR, reverse transcription quantitative polymerase chain reaction.