Figure 5. The targeting relationship between miR-218 and MITF is determined by bioinformatics prediction and a confirmatory dual-luciferase reporter gene assay. (A) the mRNA expression of MITF detected by RT-qPCR; (B) the protein expression of MITF detected by western blot assay; (C) the binding sites of miR-218 to MITF predicted by online software; (D) the targeting relationship of miR-218 and MITF determined by dual-luciferase reporter gene assay; (D) * versus the sham group, P < 0.05; # versus the MI group, P < 0.05; n = 3. Data were analyzed by one-way ANOVA. Pairwise comparison after ANOVA was performed by LSD-t.