Research Paper Volume 11, Issue 15 pp 5368—5388

Downregulation of microRNA-218 is cardioprotective against cardiac fibrosis and cardiac function impairment in myocardial infarction by binding to MITF

Figure 7. Upregulated MITF expression alleviates pathological damage of cardiac tissues in MI rats. (A) the ratio of heart to body weight, n = 6; (B) serum levels of LDH and CPK examined by the biochemical analyzer, n = 6; (C) HE staining showing pathological changes of cardiac tissues (× 200), n = 3; (D) ultrastructure of cardiomyocytes observed by transmission electron microscope (× 5000), n = 3; (E) TTC staining showing the infarct size of rats, n = 3; (F) TUNEL staining showing the apoptotic index of cardiomyocytes (× 400), n = 3; (G) Western blot assay showing protein levels of apoptosis-related factors Bax and Bcl-2 in cardiac tissues, n = 3; * versus the sham group, P < 0.05; # versus the MI group, P < 0.05; Data were analyzed by one-way ANOVA. Pairwise comparison after ANOVA was performed by LSD-t. MI, myocardial infarction; MITF, microphthalmia-associated transcription factor; LDH, lactate dehydrogenase; CPK, creatine phosphokinase.