Figure 8. Upregulated MITF expression reduces cardiac fibrosis and promotes angiogenesis in MI rats. (A) Masson staining showing the cardiac fibrosis of rats (× 200); (B) immunohistochemical staining showing the expression of type I collagen and type III collagen (× 200); (C) MVD and MMVD of cardiac tissues; (D) the mRNA expression of angiogenesis-related factor VEGF detected by RT-qPCR; (E) the protein expression of angiogenesis-related factor VEGF detected by western blot assay. * versus the sham group, P < 0.05; # versus the MI group, P < 0.05; n = 3. Data were analyzed by one-way ANOVA. Pairwise comparison after ANOVA was performed by Fisher’s least significant difference. MI, myocardial infarction; MITF, microphthalmia-associated transcription factor; MVD, microvascular density; MMVD, mean microvessels diameter; RT-qPCR, reverse transcription quantitative polymerase chain reaction; VEGF, vascular endothelial growth factor.