Research Paper Volume 11, Issue 15 pp 5368—5388

Downregulation of microRNA-218 is cardioprotective against cardiac fibrosis and cardiac function impairment in myocardial infarction by binding to MITF

Figure 9. Upregulated MITF expression inhibits oxidative stress and inflammatory injury in MI rats. (A) the levels of MDA and the activity of GSH-Px and SOD determined by the spectrocolorimetric method, n = 3; (B) the mRNA expression of IL-1β, IL-6 and TNF-α detected by RT-qPCR, n = 3; (C) the serum levels of IL-1β, IL-6 and TNF-α detected by ELISA, n = 6; * versus the sham group, P < 0.05; # versus the MI group, P < 0.05; Data were analyzed by one-way ANOVA. Pairwise comparison after ANOVA was performed by LSD-t. MI, myocardial infarction; MITF, microphthalmia-associated transcription factor; ELISA, enzyme-linked immunosorbent assay; IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; MDA, malondialdehyde; GSH-Px, glutathione peroxidase; SOD, superoxide dismutase; RT-qPCR, reverse transcription quantitative polymerase chain reaction.