Research Paper Volume 11, Issue 15 pp 5445—5462

Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells

Figure 4. Antioxidants suppress CRP-induced osteogenic signaling in HAoSMCs. (A-E) Scatter dot plots and arithmetic means ± SEM (n=6; arbitrary units, a.u.) of MMP2 (A), MMP9 (B), PAI1 (C), CBFA1 (D) and ALPL (E) relative mRNA expression in HAoSMCs treated with control (CTR) or 10 µg/ml recombinant human CRP without and with 10 µM TEMPOL or 10 µM TIRON. *(p<0.05), **(p<0.01), ***(p<0.001) significant vs. control HAoSMCs; †(p<0.05), ††(p<0.01), †††(p<0.001) significant vs. HAoSMCs treated with CRP alone.