Figure 1. Heat stress induces ROS generation and apoptosis in bovine ovarian GCs. (A, B) Intracellular accumulation of ROS at different temperatures, quantified by DCF fluorescence. Scale bars, 50 μm. (C–E) Immunoblot analysis of Bax/Bcl-2 and cleaved caspase-3in GCs. (F) Apoptosis induction by heat stress in GCs, analyzed by FACS assay. Data represent mean ± SEM; n = 3 in each group. *P < 0.05; **P < 0.01; ***P < 0.001.