Research Paper Volume 11, Issue 15 pp 5535—5547

HO-1 reduces heat stress-induced apoptosis in bovine granulosa cells by suppressing oxidative stress

Figure 2. Heat stress leads to dysfunction of the antioxidant defense system and oxidative stress in GCs. (AD) Western blotting showing the expression of Nrf2, HO-1, and SOD2. (E) Nuclear translocation of Nrf2 evaluated by DCF fluorescence. Scale bars, 50 μm. (F) Estimation of SOD activity. (G) Estimation of GSH-Px activity. (H) Nuclear translocation of Nrf2 determined by western blotting. (I) MDA content. Data represent mean ± SEM; n = 3 in each group. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.