Figure 2. Heat stress leads to dysfunction of the antioxidant defense system and oxidative stress in GCs. (A–D) Western blotting showing the expression of Nrf2, HO-1, and SOD2. (E) Nuclear translocation of Nrf2 evaluated by DCF fluorescence. Scale bars, 50 μm. (F) Estimation of SOD activity. (G) Estimation of GSH-Px activity. (H) Nuclear translocation of Nrf2 determined by western blotting. (I) MDA content. Data represent mean ± SEM; n = 3 in each group. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.