Figure 3. HO-1 gene knockdown enhances ROS generation and induces apoptosis in GCs under heat stress. (A) Western blot analysis of HO-1 expression after siRNA mediated knockdown of HO-1. (B, C) Annexin V/PI FACS analysis of apoptosis. (D–F) Expression of Bax/Bcl-2 and cleaved caspase-3 by western blot. (G, H) Intracellular ROS accumulation detected through DCF fluorescence. Scale bars, 50 μm. Data represent mean ± SEM; n = 3 in each group. *P < 0.05; **P < 0.01; ***P < 0.001.