Figure 5. Hemin-mediated HO-1 induction decreases oxidative stress and attenuates ROS generation and apoptosis in GCs exposed to heat stress. (A) qRT-PCR analysis of HO-1 gene expression in GCs pre-treated with hemin (10 μmol/L for 48h). (B, C) Effect of HO-1 overexpression on ROS accumulation in GCs under heat stress. (D, E, G, H) HO-1, SOD2, and Bax/Bcl-2 expression determined by western blotting. (F) Hemin pre-treatment reduced apoptosis of heat-stressed GCs, as determined by FACS assay. (I) Enhanced SOD activity in hemin-treated GCs. Data represent mean ± SEM; n = 3 in each group. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.