Research Paper Volume 11, Issue 15 pp 5548—5569

Ginseng increases Klotho expression by FoxO3-mediated manganese superoxide dismutase in a mouse model of tacrolimus-induced renal injury

Figure 4. Effect of KRGP administration on Klotho-induced MnSOD signaling pathway in Tac-induced oxidative stress using immunohistochemical analysis in a mouse model. Representative images of immunohistochemical analysis and its quantification for Klotho (A and B) and MnSOD (C and D), as well as in situ hybridization of MnSOD mRNA (E and F) in the tissue sections from mouse kidneys. Asterisks indicate positive renal tubules for Klotho and MnSOD. Bar = 50 μm. #P < 0.05.