Figure 4. Effect of KRGP administration on Klotho-induced MnSOD signaling pathway in Tac-induced oxidative stress using immunohistochemical analysis in a mouse model. Representative images of immunohistochemical analysis and its quantification for Klotho (A and B) and MnSOD (C and D), as well as in situ hybridization of MnSOD mRNA (E and F) in the tissue sections from mouse kidneys. Asterisks indicate positive renal tubules for Klotho and MnSOD. Bar = 50 μm. #P < 0.05.