Research Paper Volume 11, Issue 15 pp 5548—5569

Ginseng increases Klotho expression by FoxO3-mediated manganese superoxide dismutase in a mouse model of tacrolimus-induced renal injury

Figure 5. Effect of KRGP administration on Klotho-induced MnSOD signaling pathway in Tac-induced oxidative stress using immunoblot analysis in a mouse model. Representative immunoblot image (A) and its quantification of Klotho (B), PI3K (C), p-AKT/t-AKT (D), p-FoxO3a/t-FoxO3a (E), and MnSOD (F) in the renal cortex. The relative optical densities of bands in each lane were normalized to that of each band of β-actin or total form protein in the same gel. Data are presented as mean ± SE. n = 8. #P < 0.05.