Research Paper Volume 11, Issue 15 pp 5593—5612

TGFβ1- miR-140-5p axis mediated up-regulation of Flap Endonuclease 1 promotes epithelial-mesenchymal transition in hepatocellular carcinoma

Figure 1. FEN1 is up-regulated in HCC tissues and indicates poor prognosis. (A) FEN1 mRNA expression in HCC among the three studies assessed by ONCOMINE analysis. (B) Elevated expression of FEN1 in HCC tissues compared to normal tissues in the GEPIA database. (C) FEN1 IHC in HCC and para-cancer tissues. (D) Quantification of FEN1 IHC staining; n = 34 per group, **P < 0.01. (E) FEN1 IHC score; n = 34 per group, **P < 0.01. (F and G) Quantitative RT-qPCR analysis of FEN1 mRNA expression; n = 21 per group, *P < 0.05, **P < 0.01, # represents no difference. (H) RT-qPCR analysis of FEN1 mRNA expression, n = 10 for HCC with metastasis and n = 11 for HCC without metastasis, **P < 0.01. High expression of FEN1 indicated poor overall survival (I) and disease free survival (J) in the GEPIA database.