Research Paper Volume 11, Issue 15 pp 5593—5612

TGFβ1- miR-140-5p axis mediated up-regulation of Flap Endonuclease 1 promotes epithelial-mesenchymal transition in hepatocellular carcinoma

Figure 3. FEN1 promotes tumor growth and lung metastasis in vivo. (A) Images of nude mice bearing subcutaneous tumor xenografts derived either from Sh3-FEN1 (A1) or OE-FEN1 cells (A2). (B1 and B2) Xenograft tumor growth curves; n = 5 per group, *P < 0.05 and **P<0.01. (C1 and C2) Weights of tumors derived from euthanized nude mice at the endpoint; n = 5 per group, **P < 0.01. (D1 and D2) Representative images of lungs with metastatic nodules shown on the surfaces (arrows indicate the nodules) 30 days after injection of cells stably silencing or overexpressing FEN1. (E1 and E2) Representative H & E stained lung tissue sections showing metastatic nodules (arrows); original magnification, 100 x for the upper panels and 200 x for the lower panels. (F1 and F2) Weight growth curves of nude mice, n = 5 per group. (G1 and G2) Number of metastatic nodules on the lung surfaces; data represent mean ± SD, n = 5 per group, **P < 0.01.