Research Paper Volume 11, Issue 15 pp 5593—5612

TGFβ1- miR-140-5p axis mediated up-regulation of Flap Endonuclease 1 promotes epithelial-mesenchymal transition in hepatocellular carcinoma

Figure 8. TGF-β1 up-regulates FEN1 and down-regulates miR-140-5p. (A and B) Representative images showing induction of morphological characteristics after 48 h of TGF-β1 (10 ng/mL) treatment. (C and D) RT-qPCR showed that miR-140-5p mRNA was significantly decreased in HCC cells treated with TGF-β1, and FEN1 was up-regulated in TGF-β1-treated HCC cells, *P < 0.05 and **P < 0.01. (E and F) Western blot analysis of EMT markers and FEN1 in TGF-β1-treated HCC cells.