Research Paper Volume 11, Issue 15 pp 5646—5665

The long noncoding RNA MIR210HG promotes tumor metastasis by acting as a ceRNA of miR-1226-3p to regulate mucin-1c expression in invasive breast cancer

Figure 1. MIR210HG functions as a potential oncogenic lncRNA and confers a poor prognosis in invasive breast cancer patients. (A) lncRNAs microarray data of invasive breast cancer samples compared with that of normal control tissues are presented in a heat map. Red represents high expression, and green represents low expression. MIR210HG (Ensembl: ENSG00000247095) is listed on the right. (B) Heat map of differentially expressed miRNAs from invasive breast cancer miRNA arrays and miRNAs differentially expressed in miRNA arrays. miR-1226-3p (Ensembl: ENSG00000221585) is listed on the right. (C) Venn diagrams showing the number of potential non-coding RNAs targeting metastasis-related genes, as predicted by two databases: miRcode and LncBase Predicted. (D) Real-time PCR assay shown that MIR210HG is significantly up-regulated in 45 paired fresh IBC tissues. (E) Relative expression of MIR210HG in IBC patients with lymph node and distant metastasis. (F) Further analysis indicated that TNM stage was positive correlated with MIR210HG. (G and H) Kaplan-Meier plots of overall-survival (OS) and disease-free survival (DFS) in IBC patients with high and low levels of MIR210HG. *p < 0.05, **p < 0.01.