Research Paper Volume 11, Issue 15 pp 5646—5665

The long noncoding RNA MIR210HG promotes tumor metastasis by acting as a ceRNA of miR-1226-3p to regulate mucin-1c expression in invasive breast cancer

Figure 2. MIR210HG is up-regulated in breast cancer cell lines MDA-MB-231 and MCF-7, and knockdown MIR210HG inhibits cell proliferation, growth and invasion. (A) MIR210HG data set from the TCGA cell line database (The Atlas of ncRNA in cancer). (B) Real-time PCR results confirmed that MIR210HG was increased obviously in MDA-MB-231, MCF-7, 742T, 606T and MDA-MB-134 cell line. (C) shRNA-MIR210HG with high interference efficiency in 293t cell. (D) MIR210HG knockdown in MDA-MB-231 and MCF-7 transfected with shRNA was determined using qRT-PCR. (E and F) MTT assay indicated that shRNA-MIR210HG could significantly suppress the proliferation abilities of MDA-MB-231 and MCF-7 cells compared with negative control (sh-NC) group. (G) Invasion assay of MDA-MB-231 and MCF-7 cells with shRNA-MIR210HG by transwell assay. (H) Colony formation assay revealed that the silencing of MIR210HG greatly reduced the number of colonies of the MDA-MB-231 and MCF-7 cells in comparison with the sh-NC groups. *p < 0.05.