Figure 3. MIR210HG regulates miR-1226-3p by acting as a ceRNA. (A) MIR210HG colocalized with miR-1226-3p in IBC cell line MDA-MB-231 were detected by FISH assay, DAPI, 4′,6-diamidino-2-phenylindole, Magnification, × 400. (B) qRT-PCR results showed an inverse expression correlation between MIR210HG and miR-1226-3p in breast cancer tissues. (C) Diagram of wild-type (WT) and mutant (Mut) luciferase reporter plasmids, a putative miR-1226-3p target site in the 3′-UTR of MIR210HG mRNA was predicted in a bioinformatics analysis. (D) Luciferase reporter assay in human MDA-MB-231 cells co-transfected with WT and Mut type MIR210HG reporter and miR-1226-3p mimics. (E) qRT-PCR results showed that miR-1226-3p expression was lower in breast cancer cell lines (MDA-MB-231 and MCF-7 cells). (F) qRT-PCR results indicated miR-1226-3p was downregulated in IBC tissues compared with para-carcinoma groups. *p < 0.05, **p < 0.01.