Research Paper Volume 11, Issue 15 pp 5646—5665

The long noncoding RNA MIR210HG promotes tumor metastasis by acting as a ceRNA of miR-1226-3p to regulate mucin-1c expression in invasive breast cancer

Figure 5. MIR210HG promotes growth of breast tumor in murine xenograft models, and Single-cell sequencing results show that MIR210HG/MUC1 gene expression level is positively correlated with tumor metastasis pathway in invasive breast cancer. (A) Effect of subcutaneously injection of MDA-MB-231 cells transfected with sh-NC or sh MIR210HG on the tumor growth. (B and C) Knockdown of MIR210HG expression significantly inhibited breast cancer cell growth in nude mice, and the tumor volume also weight were significantly reduced in the sh-MIR210HG group compared to that in the sh-NC group. (D) Mean-centered, hierarchical clustering of MIR210HG-related genes from TCGA multiple tumors data (UCSC Cancer Genomics Browser). Both MIR210HG and MUC1 are relatively high expression in a variety of solid tumors. (E) Correlations between the gene (MIR210HG and MUC1-C) of interest and functional states in 3 different single-cell datasets (CancerSEA). Single-cell datasets sequencing results show that MIR210HG and MUC1-C are closely related to EMT and tumor metastasis pathway. (FH) Single-cell datasets of EXP0052 display 2 functional states that are significantly related to MUC1-C gene. (I) Functional relevance in different cell groups (EXP0052 single-cell datasets). Single-cell sequencing data show a significant positive correlation between MUC1-C gene expression and tumor EMT pathway regulation. (J) MUC1-C is positively associated with tumor metastasis similarly. *p < 0.05.