Research Paper Volume 11, Issue 15 pp 5646—5665

The long noncoding RNA MIR210HG promotes tumor metastasis by acting as a ceRNA of miR-1226-3p to regulate mucin-1c expression in invasive breast cancer

Figure 6. MIR210HG is related to the molecular typing of malignant metastasis of IBC, and MIR210HG/miR-1226-3p/MUC1-C axis on tumor metastasis-related EMT pathway. (AC) Using the database of MD Anderson Cancer Center (TANRIC database), we found that MIR210HG was significantly associated with ER, PR and Her2 status in invasive breast cancer patients. (D) A 50-gene qPCR assay (PAM50) was developed to identify the intrinsic biological subtypes, the luminal A (LumA), luminal B (LumB), HER2-enriched (HER2-E), basal-like, and normal-like breast cancer subtypes. Analysis of sequencing data by TCGA-BRCA database showed that MIR210HG expression level was significantly correlated with PAM50 molecular typing. (E) TCGA-BRCA database sequencing data analysis showed that MIR210HG expression levels were associated with clinical treatment sensitivity in ERPR/Her2-guided typing. (F) MUC1-C was significantly overexpressed in IBC with lymph node metastasis compared to IBC non-metastasis (scale bars’ values are shown in each microphotograph, 50 μm). (G) WB analysis of MUC1-C, SMAD2, p-ERK/T-ERK, Snail, E-cadherin, and N-cadherin in MDA-MB-231 and MCF-7 cells. *p < 0.05, **p < 0.01.