Research Paper Volume 11, Issue 15 pp 5689—5704

Up-regulation of SPC25 promotes breast cancer

Figure 3. The function of SPC25 in BC cells. (A) Single-cell analysis indicated that SPC25 is primarily involved in regulation of the cell cycle, DNA damage and repair, and proliferation in BC. (B, C) Data from Braune EB (No. cells = 369) demonstrated that SPC25 mRNA expression was positively correlated with regulation of the cell cycle, DNA damage and repair, and proliferation. (D) The Human Protein Atlas database revealed that SPC25 was colocalized with microtubule proteins in the cytoplasm of U-2 OS and U-251 MG cells. (E) SPC25 expression in BC cells. (F) Knockdown in MDA-MB-231 and MCF-7 cells with si-SPC25 or si-NC. (G) Colony formation and (H, I) CCK-8 assays demonstrated that knockdown of SPC25 suppressed proliferation of MDA-MB-231 and MCF-7 cells. (si-NC, siRNA negative control; *p<0.05, **p<0.01, ***p<0.001).