Figure 4. SPC25 may regulate TFs and BC-related genes in BC. (A) Top 20 TFs that potentially regulate SPC25 in different human cancers. (B) TFs with high regulatory potential in MDA-MB-231 and MCF-7 cell lines (10k distance to TSS). (C) CHIP-seq results for binding of FOXM1 and the SPC25 gene in MCF-7 cells. (D) Correlation between SPC25 and FOXM1 mRNA expression. (E) SPC25 DNA methylation modification in BC. (F) Correlation between SPC25 mRNA expression and DNA methyltransferase (DNMT) expression. (G) Correlation between SPC25 mRNA expression and mutation frequencies in tumor-associated genes. (TF, transcription factor; TSS, transcription start site. P-value=0, p<0.0001).