Figure 6. MiR-627-3p inhibits invasion by HOS cells in vivo. (A, B) Livers were dissected from mice and either macroscopically photographed or sectioned and stained with hematoxylin-eosin. (C) Statistical results for the number of metastases. ** P< 0.05. (D, E) Western blot and real-time PCR analyses showing the Effects of miR-627-3p agomir on expression of PTN, Cyclin D1 and MMP2 in tumor tissues dissected from livers. Data are the mean ± SEM. ** P< 0.05. (F) Schematic diagram of the regulation of PTN by miR-627-3p and its effects on osteosarcoma cell proliferation and metastasis.