Figure 5. Knockdown of ATG3 attenuates the autophagic flux induced by lonafarnib and sorafenib co-treatment. (A) and (B) Detection of autophagic flux with the mRFP-GFP-LC3 reporter in HepG2 cells in response to siRNA treatments of negative control (NC) and ATG3 under a microscope. Scale bar = 10 μm. (C) Quantification of cells with LC3 puncta as indicated. ns, P > 0.05; ***P < 0.001. (D) Western blot analysis was performed to detect changes in LC3B and cyclin D1 proteins in cells transfected with indicated siRNAs. (E) and (F) Ratio of the conversion of LC3B-I to LC3B-II (E) and expression of cyclin D1 (F). ns, P > 0.05; *P < 0.05; **P < 0.01.