Research Paper Volume 11, Issue 17 pp 6674—6690

RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways

Figure 5. Silencing of RPN2 suppressed HCC cell migration and invasion. After shRNA-RPN2 plasmid transfection, migration and invasive processes of HepG2 and Huh-7 cells were examined using injury healing (A, B) and transwell migration assays (C, D). (E, F) WB analysis detected the protein level of E-cadherin and N-cadherin in HCC cells transfected with shRNA-RPN2. The band of target protein was normalized to the density of action. The quantification was performed independently in a single band. Representative data from three separate experiments were recorded as mean ± SD. *P < 0.05.