Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 17 pp 6674—6690
RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways
Figure 8. STAT3 is involved in RPN2-regulated MMP-9 expression level in HepG2 cells. (A, B) HepG2 cells were transfected with AD-RPN2 or shRNA-RPN2 to modulate the expression of RPN2 expression. Protein expression and phosphorylation of MMP-9 and STAT3 was detected by WB. © HepG2 cells stably expressing RPN2 were treated with AG490 to block the STAT3 signal transduction. Protein expression of MMP-9 and STAT3 were then analyzed by WB. The band of target protein was normalized to the density of action. The quantification was performed independently in a single band. The experiments were performed three times. Data are represented as mean ± SD.