Research Paper Volume 11, Issue 17 pp 6674—6690

RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways

Figure 9. NF-κB is partially responsible for RPN2-mediated MMP-9 expressive level in HepG2 cells. (A, B) HepG2 cells were transfected with AD-RPN2 or shRNA-RPN2 to modulate the RPN2 level, WB assay was conducted to examine the MMP-9 as well as NF-κB protein expression. (C, D) mRNA expression of MMP-9 and NF-κB p65 was then analyzed by qPCR. (E) HepG2 cells stably expressing RPN2 were transfected with shRNA-p65 to silencing the p65 expression. Protein expression of MMP-9 and p65 were then determined via WB. The band of target protein was normalized to the density of action. The quantification was performed independently in a single band. The experiments were performed three times. Data are represented as mean ± SD. *P < 0.05, ***P < 0.001 vs control group.