Research Paper Volume 11, Issue 17 pp 6691—6713

PU-91 drug rescues human age-related macular degeneration RPE cells; implications for AMD therapeutics

Figure 1. PU-91 regulates the mitochondrial biogenesis pathway. We used quantitative qRT-PCR to measure the relative mtDNA copy number (A), and the gene expression of markers of the mitochondrial biogenesis pathway such as PGC-1α (B), NRF-1 (C), NRF-2 (D), PPAR-α (E), and PPAR-γ (F). PU-91-treated AMD cybrids (AMD PU-91) had higher mtDNA copy numbers and increased gene expression levels of all the above-mentioned markers (p≤0.05, n=4-5). Data are presented as mean ± SEM and normalized to untreated (UN) AMD cybrids which were assigned a value of 1. Mann-Whitney test was used to measure statistical differences; *p≤0.05.