Research Paper Volume 11, Issue 17 pp 6691—6713

PU-91 drug rescues human age-related macular degeneration RPE cells; implications for AMD therapeutics

Figure 4. PU-91 regulates apoptotic cell death. qRT-PCR analysis showed downregulation of apoptotic genes such as Caspase-3 (p≤0.05, n=4) (A) and BAX (p≤0.05, n=4) (B) in AMD cybrids treated with PU-91. Using the MTT assay, it was observed that PU-91-treated AMD cybrids had a higher number of viable cells compared to the untreated group (p≤0.05, n=4) (C). Data are presented as mean ± SEM and normalized to untreated (UN) AMD cybrids which were assigned a value of 1. Mann-Whitney test was used to measure statistical differences; *p≤0.05, **p≤0.01.