Research Paper Volume 11, Issue 17 pp 6714—6733

MeCP2 inhibits cell functionality through FoxO3a and autophagy in endothelial progenitor cells

Figure 1. Identification of EPCs from human umbilical cord blood. (A) EPCs at passage 1 (P1) exhibited monolayer growth and cobblestone morphology after 2 weeks. (B) Cells were characterized by the immunofluorescence detection of CD31, CD34, VWF, VEGFR2, and CD133. (C) Flow cytometry revealed the positive expression rates of CD34, VEGFR2, and CD133. (D) Uptake of ac-LDL and binding of FITC-UEA-1 are characteristic functions of endothelial cells.