Research Paper Volume 11, Issue 17 pp 6714—6733

MeCP2 inhibits cell functionality through FoxO3a and autophagy in endothelial progenitor cells

Figure 4. MeCP2 inhibited FoxO3a, autophagy, and cellular function. (A) Protein levels of FoxO3a, LC3 II, Beclin1, p62, ATG5, and ATG7 were detected by western blotting after transfection with Ad-MeCP2 or Ad-sh-MeCP2 for 48 h. (B) Cell migration was evaluated by Transwell migration assays after transfection with Ad-MeCP2 or Ad-sh-MeCP2 for 48 h. (C) Cell adhesion ability was evaluated by matrix adhesion assays after transfection with Ad-MeCP2 or Ad-sh-MeCP2 for 48 h. (D) Angiogenic ability was evaluated by Matrigel assays after transfection with Ad-MeCP2 or Ad-sh-MeCP2 for 48 h. (E) Cell viability was evaluated with CCK-8 after transfection with Ad-MeCP2 or Ad-sh-MeCP2 for 48 h. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control.