Figure 3. Combined treatment with sodium cantharidate and erlotinib enhances growth and migration inhibition and promotes apoptosis of osteosarcoma cells. (A). MG63 and U2OS cells were treated with DMSO, SC, erlotinib, or SC plus erlotinib for the indicated times, and the MTT assay was performed to determine cell viability. Apoptosis analysis (JC-1 staining) was conducted in MG63 (B) and U2OS (C) cells treated as above. Migration assay results in MG63 (D) and U2OS (E) cells 24 h after individual or combined drug treatment. Magnification, 200x. Error bars indicate SD. **p < 0.01 vs. DMSO. ##p < 0.01 vs. SC. ^^p < 0.01 vs. erlotinib; n = 3. Sc, sodium cantharidate; Er, erlotinib.