Research Paper Volume 11, Issue 17 pp 6734—6761

MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer

Figure 2. MiR-330-3p regulated proliferation, apoptosis and cell cycle of NSCLC cells. (A) The proliferative ability of A549 and HCC827cells after transfection was evaluated by MTT assay. Data represent mean ± SD. (B, C) The apoptosis of A549 and HCC827 cells was determined by Annexin V-fluorescein isothiocyanate (FITC)/7-amino-actinomycin D (7-AAD) staining. The percentages of Annexin-V-positive cells were indicated. The expression of Bax and Bcl-2 was determined by western blotting in A549 and HCC827 cells. GAPDH was used as a loading control. (D, E) The cell cycle was analyzed by flow cytometry after PI staining, and the data were processed with ModFit LT program. Western blotting of cyclin D1 was shown under each band. Protein level quantification was normalized to GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001.