Research Paper Volume 11, Issue 17 pp 6734—6761

MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer

Figure 3. MiR-330-3p promoted tube formation, cell migration and invasion in A549 and HCC827 cells. (A) Tube formation assay measuring proangiogenic activity in A549 and HCC827 cells; tube formation was assessed using an inverted light microscope (Olympus IX71, original magnification ×100). (B) Wound-healing assays were performed to assess NSCLC cell migration. Wound closure was determined 24 h after the scratch. (C) Transwell migration assay measuring NSCLC cell migration in A549 and HCC827 cells stably transfected with NC-LV, OE-miR-330-3p-LV or anti-miR-330-3p-LV, respectively. (D) Transwell invasion assay was used to quantify cell invasion in a Matrigel-coated chamber. The average number of cells migrating per field of view in three different experiments is plotted. *P < 0.05, **P < 0.01.