Research Paper Volume 11, Issue 17 pp 6734—6761

MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer

Figure 4. MiR-330-3p promoted angiogenesis, cell migration and invasion of HUVEC cells co-cultured with A549 and HCC827 cells. (A) HUVECs were seeded on top of extracellular matrix, in the presence of conditioned medium (CM) obtained from NC-LV, OE-miR-330-3p-LV or anti-miR-330-3p-LV-transfected A549 and HCC827 cells. Tube formation was assessed using an inverted light microscope (original magnification ×100). (B) HUVECs were seeded in 6-well plates, in the presence of CM obtained from NC-LV, OE-miR-330-3p-LV or anti-miR-330-3p-LV transfected A549 and HCC827 cells. Wound closure was determined 6 h and 12 h after the scratch. (C) Cell invasion was evaluated using a 24-transwell chamber with 8-μm pore insert. HUVECs were seeded in the upper chamber, in the presence of CM obtained from NC-LV, OE-miR-330-3p-LV or anti-miR-330-3p-LV-transfected A549 and HCC827 cells. The migrated cells were crystal violet-stained and assessed using an inverted light microscope (×100). (D) The expression of VEGFA by western blotting. *P < 0.05, **P < 0.01.