Figure 7. GRIA3 is a direct target of miR-330-3p. (A) Relative luciferase activity of 293T cells after co-transfection with wild type (Wt) or mutant (Mt) GRIA3 3′ UTR reporter genes and miR-330-3p mimics or control. (B) Expression of GRIA3 mRNA in A549 and HCC827-transfected cells was determined by qRT-PCR. (C) The expression of GRIA3 was analyzed in A549 and HCC827 transfected cells by western blotting. (D) Expression of GRIA3 mRNA in peripheral blood from 62 newly diagnosed BM+ and 60 newly diagnosed BM- NSCLC patients using qRT-PCR. (E) Kaplan-Meier plots with log rank test for overall survival and relapse-free survival of lung adenocarcinoma cancer patients with high GRIA3 expression and low GRIA3 expression, respectively. Data represent mean ± SD, *P < 0.05, **P < 0.01. P value was calculated by one-way ANOVA.