Research Paper Volume 11, Issue 18 pp 7357—7385

miR-375-3p suppresses tumorigenesis and partially reverses chemoresistance by targeting YAP1 and SP1 in colorectal cancer cells

Figure 1. Downregulation of miR-375-3p associated with chemoresistance, malignancy and poor prognosis. (A) The association of miR-375-3p expression and 5FU-resistance were measured by qRT-PCR in CRC parental cell lines (HCT116, HCT8) and 5FU-resistant cell lines (HCT116/FU, HCT8/FU). (B) The miR-375-3p expression in CRC cell lines (HCT116, HT29, HCT8, SW480, SW620, DLD1 and CaCO2) were compared with that in the colonic mucosal epithelial cell (FHC) by qRT-PCR. (C) The association of miR-375-3p expression and 5FU-resistance were measured by qRT-PCR in 5FU-sensitive and 5FU-reisistant groups. MiR-375-3p expression was reduced in 5FU-reisistant group. (D, E) qRT-PCR analysis of miR-375-3p expression in CRC tissues compared with that in adjacent normal tissues from our clinical samples (n = 40, respectively)and Starbase v3.0 database. MiR-375-3p expression was reduced in CRC tissues. (F) The association analysis of miR-375-3p expression with TNM stage (I, II, III, IV) in CRC patients from TCGA database are shown. (G) Kaplan-Meier survival curves for miR-375-3p expression in associated with overall survival based on our clinical samples (n =130, log-rank test, p < 0.001). (H) Kaplan-Meier survival curves for miR-375-3p expression in associated with overall survival from TCGA database. * P < 0.05, ** P < 0.01 and *** P < 0.001.