Research Paper Volume 11, Issue 18 pp 7357—7385

miR-375-3p suppresses tumorigenesis and partially reverses chemoresistance by targeting YAP1 and SP1 in colorectal cancer cells

Figure 4. The expression of YAP1 and SP1 are inversely associated with miR-375-3p and chemosensitivity. (A) Potential target genes of miR-375-3p were predicted in three bioinformatics databases. (B) The mRNA expressions of 13 candidate targets were analyzed in HCT116/FU cells transfected with miR-375-3p/NC mimics. (C, D) The mRNA expression levels of YAP1 and SP1 were analyzed in CRC patients (n=40). (E, F) qRT-PCR analysis of the expressions of YAP1 and SP1 in 5FU-resistant and 5FU-sensitive groups (n=30, respectively). (G, H) The correlations of miR-375-3p expression and YAP1/SP1 expressions were analyzed in CRC tissues, respectively (n = 25). *P < 0.05, **P < 0.01 and ***P < 0.001.