Figure 5. Quantification of pro-hypertrophic myocardial proteins by western blot. Bar graphs illustrating Akt (A), p-Akt (B), mTOR (C), p-mTOR (D), p38 MAPK (E), and p-p38 MAPK (F) protein levels quantified by western blot in the myocardium of control sham (n=8), AAV8-LDLr sham (n=8), control TAC (n=8), and AAV8-LDLr TAC (n=8) mice 8 weeks after operation. All protein levels were normalized to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein level. The GAPDH/ß-tubulin ratio is shown in panel g. Data are expressed as means ± SEM (n=8). Representative images of western blots are shown in panel h.